Use of Spermac~(R) Staining Technique in the Determination of Acrosomal Defects in Cat Semen

摘要

The types and rates of acrosome and other (head mid-piece and tail) abnormal spermatozoon types were determined by the Spermac~(R) staining technique. Semen from 5 stray torn cats under the same management conditions was used Semen collection was performed under general anesthesia by electro-ejaculator once a week for 5 weeks After ejaculation the semen was diluted by 100 μl of 0 9% NaCI solution and stained with Spermac(r) stain for morphological evaluation. The morphological criteria were acrosome, other (head mid-piece and tail) and total morphological defect rates and were 9.20 ± 2 55% 9 20 ± 3 49% and 18 40 ± 4 79%. respectively. It was concluded at the end of the study that the Spermac~(R) staining technique could provide a detailed observation of cat spermatozoa especially the acrosomal region and could be employed in the determination of acrosomal defects.