Anticancer activities of a chemically sulfated polysaccharide obtained from Grifola frondosa and its combination with 5-Fluorouracil against human gastric carcinoma cells

摘要

Chemically sulfated polysaccharide(S-GAP-P)was derived from water-insoluble polysaccharide of Grifola frondosa mycelia.In this research,we investigated the anticancer effects of S-GAP-P and its combination with 5-fiuorouracil(5-FU)on human gastric carcinoma SGC-7901 cells.Results showed that S-GAP-P distinctly inhibited SGC-7901 cells growth in a dose-dependent manner and induced cell apoptosis evidenced by characteristic DNA ladder and sub-G0/G1 peak.Furthermore,the combination of S-GAP-P(10-50 mu g/ml)with 1 mu g/ml 5-FU resulted in a significant inhibition on SGC-7901 cells growth,meaning the beneficial interaction between the two drugs.All these results suggested that S-GAP-P has evident anticancer activity through apoptotic induction and could significantly accelerate the anticancer activity of 5-FU.