cDNA and gene structures of two phospholipase A(2) isoforms, acidic PLA(2) PA4 and PLA(2) PA3A/PA3B/PA5, in Nemopilema nomurai jellyfish venom

摘要

We have shown that Nemopilema nomurai jellyfish venom (NnV) contains various kinds of proteolytic enzyme activities, including phospholipase (PLA), metalloproteinase (MP) and hyaluronidase activities. In this study, we reported the full-length cDNA and gene sequences of two PLA(2) isoforms: acidic PLA(2) PA4 and PLA(2) PA3A/PA3B/PA5. The full-length cDNA of acidic PLA(2) PA4 contains 483 nucleotides (nt), which encode 160 amino acids (and the stop codon), including a signal peptide, six cysteine residues that form disulfide bonds, and metal-binding and catalytic active sites. The gene sequence of the acidic PLA(2) PA4 is 1667 base pairs (bp) long and encodes three exons and two introns. The 5' donor (GT) and 3' acceptor (AG) splice sites are highly conserved. The PLA(2) PA3A/PA3B/PA5 gene contains 1366 bp, and the 498 nt of the mature mRNA encode 165 amino acids (and the stop codon). The protein includes a signal peptide, six cysteine residues that form disulfide bonds, and metal-binding and catalytic active sites. The three exons and two introns also have highly conserved donor and acceptor splice sites. InterProScan predicted PLA(2) activity domains in both isoforms. These results extend our understanding of the PLA(2) venom of the N. nomurai jellyfish and will facilitate further research. (C) 2016 Elsevier Ltd. All rights reserved.