首页> 外文期刊>Toxicon: An International Journal Devoted to the Exchange of Knowledge on the Poisons Derived from Animals, Plants and Microorganisms >Microcystin (-LR) affects hormones level of male mice by damaging hypothalamic-pituitary system.
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Microcystin (-LR) affects hormones level of male mice by damaging hypothalamic-pituitary system.

机译:微囊藻毒素(-LR)通过破坏下丘脑-垂体系统影响雄性小鼠的激素水平。

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摘要

Microcystin (-LR) (MC-LR) is a broad distributed hepatotoxic microcystin produced by common freshwater phytoplankton species. Recently, its toxic effects on male reproductive system drew great attention. To investigate its reproductive toxicity and the underlying mechanism, BALB/c mice were exposed to MC-LR by intraperitoneal injection with different injection duration (1, 4, 7 and 14 days) and injection concentration (3.75, 7.5, 15 and 30 micro g kg b.w.-1 day-1) as an in vivo test. For in vitro test, isolated mice Leydig cells were exposed to MC-LR at doses of 1, 10, 100, 250, 500 750 and 1000 nmol/L for 24 h. MC-LR did not induce significant changes in Kiss-1, GPR54, Gnrhr, Fshr or Lhr expression. It decreased the Gnrh expression in a dose- and duration-dependent manner. It increased and subsequently decreased the expressions of FSH, LH and testosterone. In in vivo test, MC-LR was not able to enter Leydig cells and had no cytotoxicity on Leydig cells. The results showed that MC-LR affected male mice serum hormones and mRNA expressions by damaging the hypothalamic-pituitary systems.
机译:微囊藻毒素(-LR)(MC-LR)是由常见的淡水浮游植物产生的分布广泛的肝毒性微囊藻毒素。最近,它对雄性生殖系统的毒性作用引起了极大的关注。为了研究BALB / c小鼠的生殖毒性及其潜在机制,采用不同注射时间(1、4、7和14天)和注射浓度(3.75、7.5、15和30 micro g)通过腹膜内注射将BALB / c小鼠暴露于MC-LR kg bw -1 天 -1 )作为体内测试。对于体外测试,将分离的小鼠Leydig细胞以1、10、100、250、500 750和1000 nmol / L的剂量暴露于MC-LR 24小时。 MC-LR不会在 Kiss-1 , GPR54 , Gnrhr , Fshr 或中引起显着变化Lhr 表达。它以剂量和持续时间依赖的方式降低了 Gnrh 的表达。它增加并随后降低了FSH,LH和睾丸激素的表达。在体内测试中,MC-LR无法进入Leydig细胞,并且对Leydig细胞没有细胞毒性。结果表明,MC-LR通过损伤下丘脑-垂体系统影响了雄性小鼠的血清激素和mRNA表达。

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