Rapid detection of microcystins in cells and water. (Special Issue: Harmful algal blooms and natural toxins in fresh and marine waters - exposure, occurrence, detection, toxicity, control, management and policy.)

摘要

Global occurrence and concern about microcystin contamination in water has prompted the development of a range of detection methods for their identification and quantification. However, most protocols are relatively time consuming, expensive and require laboratory expertise. The production of robust and sensitive recombinant antibodies has facilitated the development of a lateral flow immunoassay (ImmunoStripReg.) which can rapidly detect microcystins and nodularins in the field with minimal equipment or processing. Here we evaluate the sensitivity and cross-reactivity of the commercially produced ImmunoStripReg. and apply them to the detection of microcystins in laboratory cultures and natural samples. It was observed that while the ImmunoStripReg. are marketed for the detection of 10 micro g/l microcystin, all 7 microcystins and nodularin that were tested were detected below 1 micro g/l. Furthermore, microcystins and nodularins were successfully detected in a range of laboratory cultures and samples from irrigation ponds.