ALPHA(2)-MACROGLOBULIN OF RABBITS INHIBITS THE HABUTOBIN ACTIVITY

摘要

We have investigated whether alpha(2)-macroglobulin (alpha(2)M) of rabbits inhibits the activity of habutobin, a thrombin-like enzyme from Trimeresurus flavoviridis venom. Rabbit alpha(2)M was purified with ultracentrifugation, gel filtration on Sepharose 6B and ion exchange chromatography on DEAE-Sephacel. Inhibitory effects of rabbit alpha(2)M on habutobin was determined by the fibrin forming activity, digestion of A alpha chain of fibrinogen, and the release of fibrinopeptide A from fibrinogen. As a results, purified alpha(2)M showed a single band with high molecular weight, around 800,000 mol. wt by means of polyacrylamide gel electrophoresis using PhastSystem(R). Besides inhibiting amidolytic and caseinolytic activity of porcine trypsin, it has inhibited the activity of habutobin: that is, in the presence of rabbit alpha(2)M, fibrin forming activity of habutobin was decreased and habutobin-induced digestion of A alpha chain was inhibited. In addition, rabbit alpha(2)M reduced habutobin-induced release of fibrinopeptide A from rabbit fibrinogen. Copyright (C) 1996 Elsevier Science Ltd [References: 23]