The activation of Epimedium polysaccharide-propolis flavone liposome on Kupffer cells

摘要

Epimedium polysaccharide-propolis flavone liposome (EPL), a potent immunological pharmaceutical preparation, was investigated for the immunomodulatory activity on Kupffer cells (KCs) in vitro. The results showed that EPL could significantly induce the secretion of chemokines (RANTES and MCP-1), promote the production of nitric oxide and induced nitric oxide synthase, improve the pinocytic and phagocytic activity of KCs, promote the mRNA expression of TNF-alpha and IL-1 beta, and enhance the expression of costimulatory molecules (CD11 b and CD68) in KCs compared with Epimedium polysaccharide-propolis flavone (EP) at 30-7.5 mu g/mL. In addition, the abilities of KCs on stimulating lymphocytes proliferation and antigen presenting were significantly enhanced after stimulated with EPL compared with EP. These results suggested that EPL could activate KCs and possessed the stronger immunomodulatory effect, which provided the theoretical basis for further studying the mechanism of EPL on improving the immune response. (C) 2015 Elsevier Ltd. All rights reserved.