Surface modification of polystyrene biochip for biotin-labelled protein/streptavidin or neutravidin coupling used in fluorescence assay

摘要

A polystyrene biosensing chip to be used as a medical diagnostic tool has been developed. Its surface functionalization is optimized to bind the bioanalytes with high efficiency. Coatings of allyldextran monolayers were carried out on polystyrene chips activated with gamma-irradiation. The effect of the concentration of allyldextran solution on the coating efficiency was studied by surf ace-sensitive x-ray photoelectron spectroscopy (XPS) and contact-angle measurements. In a second step, sodium periodate chemistry was applied to functionalize the dextran layer, followed by coupling with streptavidin or neutravidin. Copyright (C) 2004 John Wiley Sons, Ltd.