Effects of toluene inhalation exposure on olfactory functioning: Behavioral and histological assessment.

摘要

Exposure to pollutant or toxic substances is known to induce adverse health effects but few studies have been devoted to study the impact on olfactory functioning although neuroreceptors in the nasal cavity are directly in contact with volatile molecules. Thus, this work was designed to evaluate in mice the potential modifications of the olfactory functioning during (1 month) and after (1 month) a prolonged toluene exposure at both sensitive/perceptive and cellular levels. Mice were exposed to 1000ppm of toluene for 5h/day, 5days/week for 4 weeks. Firstly, behavioral evaluation (T-maze test) to toluene sensitivity showed a constant decrease during all the 4 weeks of exposure (W1-W4) which continued during 2 weeks after the exposure (W5, W6). In contrast, during the last 2 weeks of the experiment (W7, W8), the sensitivity of mice to toluene went back to normal. Secondly, structural modifications, i.e. density of cells and thickness of olfactory epithelium were observed soon after the outset of exposure.The number of cells did not change at the beginning of exposure (W1, W2), decreased markedly later (W3, W4), increased significantly the first week of the recovery period (W5) and stayed stable during the following weeks (W6-W8). Concerning the thickness of neuroepithelium, the results at W1 showed a decrease followed by an increase suggesting an inflammatory process (W2, W3). In contrast, the results of W4 revealed an abrupt decrease of the thickness whereas the return to normal arose immediately at the outset of recovery period.