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Endoscopic approaches to manage in vitro and in vivo embryo development: Use of the bovine oviduct

机译:内窥镜管理体外和体内胚胎发育的方法:牛输卵管的使用

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摘要

The oviduct plays a major part in different reproductive processes providing the microenvironment for numerous steps in early embryogenesis. Consequently, there is a growing demand to perform comparative studies focusing on causal mechanisms related to embryo development within its environment including complex and holistic strategies. However, the routine flushing and transfer procedure of bovine embryos is limited to the morula and blastocyst stage. Additionally, the use of in vitro production of bovine embryos provides access to an extra amount of embryos at various stages. But the quality of these embryos does not reflect the quality of its ex vivo counterparts. For two decades our own studies have focused on use of the oviductal environment of different species to optimize early embryo development for different purposes. The current article briefly highlights some main characteristics of the fallopian tube and reviews the endoscopic approach to access the fallopian tube using the stepwise minimal invasive technique established in different species.
机译:输卵管在不同的生殖过程中起主要作用,为早期胚胎发生的许多步骤提供了微环境。因此,人们越来越需要进行比较研究,重点放在其环境中与胚胎发育有关的因果机制上,包括复杂的整体策略。但是,牛胚胎的常规冲洗和转移程序仅限于桑ula和胚泡期。另外,使用牛胚胎的体外生产提供了在各个阶段获得额外数量的胚胎的途径。但是这些胚胎的质量不能反映其离体胚胎的质量。在过去的二十年中,我们自己的研究集中在利用不同物种的输卵管环境优化不同目的的早期胚胎发育。本文简要介绍了输卵管的一些主要特征,并回顾了使用在不同物种中建立的逐步微创技术的内窥镜接近输卵管的方法。

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