Effect of cysteamine during in vitro maturation on buffalo embryodevelopment

摘要

The purpose of this study was to evaluate whether the addition of cysteamine during in vitro maturation (IVM) of buffalo oocytes enhances embryo development. Cumulus-oocyte complexes (COC) from slaughterhouse ovaries were matured in vitro in TCM 199 supplemented with 10% fetal calf serum (FCS), 0.5 mug mL(-1) FSH, 5 mug mL(-1) LH, I mug mL(-1) 17 beta estradiol and 0 (control), 50, 100 or 200 mu mol L-1 of cysteamine for 24 hours. The matured oocytes then were fertilized and cultured for 7 days.