Detection of Sheep Pox Antibodies in Different Areas of Sudan Using Agar Gel Immune-Diffusion Test (AGIO) and Passive Haemagglutination Test (PHA).

摘要

Seroprevalence of sheep pox disease in five different areas of Sudan was detected using agar gel immuno-diffusion test (AGID) and passive haemagglutination test (PHA). A total of 471 sera collected from El-obaid (North Kordofan 261), Gibaish (West Kordofan 59), Buram (South Darfur 80), Port Sudan (Red Sea 54) and Shendi (River Nile 17) were examined for sheep pox antibodies using AGID and PHA tests. Out of 471 samples tested, 420 (89.2%) were positive by AGID, while 374 (79.4%) were positive using PHA. High prevalence of sheep pox antibodies was detected in all areas studied by both techniques, higher prevalence (96.3%, 92.5%) was observed in Buram, (96.3%, 42.6%) in Red Sea, (94.1%, 52.9%) in River Nile, (89.8%, 96.9%) in Gibaish and (85.1%, 80.8%)in El obaid, respectively. Out of 24 samples collected from Gibaish, tested by AGID only, 21 were positive, while 50 of 55 samples collected from Shendi were also AGID positive for sheep pox antibodies, Most of AGID positive samples (89.5%) and PHA positive samples (88%) had 1 log2-5 log2 titers. The results indicated the high prevalence of sheep pox disease in all the studied areas and revealed that both techniques could be used for detecting sheep pox antibodies under Sudan condition.