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Salt Tolerance Screening in Six Maize (Zea mays L.) Genotypes using Multivariate Cluster Analysis

机译:使用多元聚类分析筛选六种玉米(Zea mays L.)基因型的耐盐性

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摘要

The effective indices for salt tolerant classification of maize genotypes were investigated. Six maize cultivars were treated with either 0 (control) or 200 mM NaCI (salt stress). Chlorophyll a (Chl_a), chlorophyll b (Chl_b), total chlorophyll (TC) and total carotenoid (C_(x+c)) concentrations in the salt-stressed leaves dropped significantly. Chl_a degradation in maize genotypes was correlated with maximum quantum yield of PSII (F_v/F_m). TC content was positively related to photon yield of PSII (PHI_(psll)). F_v/F_m, PHI_(psll) and photochemical quenching (qP) in the salt-stressed leaves decreased dramatically, related to the reduced net photosynthetic rate (P_n), leading to growth reduction. The chlorophyll a fluorescence parameters, F_v/F_m, PHI_(psll) and qP, in the leaf tissues decreased, while non-photochemical quenching (NPQ) increased. Data relating to pigment degradation, chlorophyll a fluorescence diminution, photosynthesis reduction and growth inhibition in saltstressed maize cultivarswere subjected to hierarchical cluster analysis using SPSS software. Two classes of genotypes were identified: salt-tolerant (Waxy 1, Waxy 2 and Waxy 3) and salt-sensitive (Honey, Sweet 1 and Sweet 2).
机译:研究了玉米基因型耐盐分类的有效指标。用0(对照)或200 mM NaCl(盐胁迫)处理6个玉米品种。盐胁迫叶片中的叶绿素a(Chl_a),叶绿素b(Chl_b),总叶绿素(TC)和总类胡萝卜素(C_(x + c))浓度显着下降。玉米基因型中的Chl_a降解与PSII的最大量子产量(F_v / F_m)相关。 TC含量与PSII(PHI_(psll))的光子产量呈正相关。盐胁迫叶片中的F_v / F_m,PHI_(psll)和光化学猝灭(qP)显着降低,与净光合速率(P_n)的降低有关,从而导致生长减少。叶组织中的叶绿素a荧光参数F_v / F_m,PHI_(psll)和qP减少,而非光化学猝灭(NPQ)增加。使用SPSS软件对盐胁迫玉米品种中的色素降解,叶绿素a荧光减弱,光合作用减少和生长抑制相关的数据进行了层次聚类分析。确定了两类基因型:耐盐性(Waxy 1,Waxy 2和Waxy 3)和盐敏感性(Honey,Sweet 1和Sweet 2)。

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