Transport and Sorting of the Solanum tuberosum Sucrose Transporter SUT1 Is Affected by Posttranslational Modification (vol 20, pg 2497, 2008)

摘要

The electrophysiological characterization of Zm SUT1 activation by oxidized glutathione (GSSG), as shown in Figure 4B of Krügel et al. (2008), was due to a change in pH value caused by the application of redox reagents to the medium. Here, we show that at constant pH, the application of GSSG has no affect on sucrose transport in oocytes (Figure 9A). DTT has only a minor inhibitory effect on sucrose uptake without any change of pH value. The Zm SUT1-mediated sucrose-induced currents were much higher at pH 3.5 than at pH 5.5 (Figure 1B), consistent with this new interpretation of our results.