Mechanical and biocompatible characterizationof a cross-linked collagen-hyaluronicacid wound dressing

摘要

Collagen scaffolds have been widely employed as a dermal equivalent to induce fibroblast infiltrations and dermalregeneration in the treatment of chronic wounds and diabetic foot ulcers. Cross-linking methods have been developedto address the disadvantages of the rapid degradation associated with collagen-based scaffolds. To eliminate thepotential drawbacks associated with glutaraldehyde cross-linking, methods using a water soluble carbodiimide havebeen developed. In the present study, the glycosaminoglycan (GAG) hyaluronic acid (HA ), was covalently attached toan equine tendon derived collagen scaffold using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) to createntSP ONGE. The HA was shown to be homogeneously distributed throughout the collagen matrix. In vitro analyses of thescaffold indicated that the cross-linking enhanced the biological stability by decreasing the enzymatic degradation andincreasing the thermal denaturation temperature. The material was shown to support the attachment and proliferationof mouse L929 fibroblast cells. In addition, the cross-linking decreased the resorption rate of the collagen as measured inan intramuscular implant model in rabbits. The material was also shown to be biocompatible in a variety of in vitro andin vivo assays. These results indicate that this cross-linked collagen-HA scaffold, ntSP ONGE, has the potential for use inchronic wound healing.