The presence of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) during meiosis improves porcine 'oocyte competence' as determined by early embryonic development after in-vitro fertilization.

摘要

The presence of purified ovine tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in culture medium after in vitro fertilization did not affect the percentage of embryos cleaving or developing to the blastocyst stage respectively 48 h or 6 daysafter insemination. In a further experiment, various concentrations (1.25, 2.50 and 5.00 鎔/ml) of purified ovine TIMP-1 were added to the culture medium for the second half (24 h) of oocyte maturation The percentage of oocytes that cleaved normally (64?, 57? and 56? respectively) was higher (P<0.05) than controls without TIMP-1 (43?). Corresponding percentages of subsequent development to the blastocyst stage were also increased (P<0.05) (34?, 32? and 28? vs. 22? in controls). There was no difference in the percentage (53?) of oocytes fertilized normally. The results indicate that the presence of TIMP-1 during the second half of in vitro oocyte maturation increased the competence of porcine oocytes to develop to the 2- to 4-cell stages afterin vitro fertilization, consequently increasing the percentage of blastocysts.