Genetic diagnosis of claudin-16 deficiency and sex determination in bovine preimplantation embryos

摘要

The reaction conditions consisted of initial denaturing at 95 C for 15 min followed by 45 cycles of 97 C for 30 sec, 56 C for 90 sec and 72 C for 30 sec.should have appeared as follows:The reaction conditions consisted of initial denaturing at 95 C for 15 min followed by 45 cycles of 94 C for 30 sec, 56 C for 90 sec and 72 C for 30 sec.