Germinal vesicle stages in pig follicular oocytes collected by different methods.

摘要

In the first of 2 experiments, oocytes were collected from follicles (4-6 mm) of prepubertal gilts by aspiration with a syringe or by dissection. After aspiration, some oocytes with compact cumulus and granulosa cells were selected. In the 2nd experiment, oocytes were collected from follicles (4 mm, 5-6 mm and 7-8 mm) of gilts on the 18th-19th day of the oestrous cycle. Germinal vesicle (GV) stages of oocytes were examined and categorized into GV1, GV2, GV3 and GV4. In oocytes collected by aspiration in the 1st experiment, a significantly lower percentage (45%) was arrested at the GV1 stage than in the case of oocytes from dissected follicles (78%). However, after morphological selection of oocytes, the percentage (58%) significantly increased. Inthe 2nd experiment, significantly higher percentages (86-90%) of oocytes from follicles of 5-8 mm were at the GV1 stage than oocytes from follicles of 4 mm (24%) and atretic follicles (57%).