Analysis of quantitative trait loci (QTL) related to rice alkali digestion value and amylose content

摘要

This study was conducted to analyze quantitative trait loci (QTL) of alkali digestion value (ADV) and amylose content. The recombinant inbred lines (RILs) were developed by single seed descent (SSD) method of progenies derived from the cross between Japonica/Tongil hybrid cultivar, Nonganbyeo, and indica elite line, BG276. The 272 individual plants of F_7 generation were used for DNA analysis by simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) techniques to construct the molecular linkage map on which the loci of the QTLs governing the characters were determined. The total length of the 12 linkage groups spaned to 1,234 cM and its average length between the markers was 9.4 cM. A total of 7 significant QTL governing 2 characters was detected with the number variation of 3 to 4 QTL for each character. For the characters controlling the grain quality and cooking behavior, a total of 4 QTL for ADV was located in chromosome 3, 7, 8, and 11, and 3 QTL for amylose content of grains in chromosome 1, 6, and 11. The phenotypic variation of respective characters explained by these QTL markers were 70% for ADV and 79.5% for amylose content of grains.