Development and Application of Weonhyeong Strain-specific SCAR Marker inPleurotus ostreatus

摘要

Weonhyeong is one of important commercial strains. It has good characteristics of bundle formation, grey colored pilei and high productivity. We previously reported grouping of 70 strains of Pleurotus ostreatus in which one group contained 35 strainsincluding Weonhyeong. Four strains in that group showed same profiles implicating no variety distinction for mushroom cultivation. Now we developed a specific marker for identification of Weonhyeong. Sequence Characterized Amplified Region (SCAR) markerwas developed from the RAPD amplicon. SCAR marker 'S-0P05' produced only one band specific to 2183, 2240, 2595 and 2725 strams showing similar banding patterns to Weonhyeong in RAPD-PCR results. The sequence of 'S-0P05' marker was unknown when comparedwith the data in the Genbank using BLASTN. BLASTX results indicated that the marker showed significant alignment with the protein sequences in Tricholoma bakamatsutake reverse transcriptase. The results indicate that this new SCAR marker ('S-0P05') willbe valuable to distinguish the Weonhyeong similar strains from Pleurotus spp.