Production of Pleurotus ostreatus and Flammulina velutipes using liquid spawn inoculation system

摘要

This research aimed at developing the efficient method and device applicable to the inoculation of mushroom using (spawn was intended to utilize) liquid spawn for the stable production of mushroom. For the mass production of liquid culture, optimal inoculum volume and cultural period were 5-6 percent and 4 days for Flammulina velutipes and 4 percent and 5 days for Pleurotus ostreatus. Fruit body weight in 850 ml polythene bottle was highest at 6 percent or 15 ml liquid inoculum for P. ostreatus and4-6 percent or 10-15 ml for F. velutipes. Weight of fruit body by the application of liquid spawn inoculation system increased up to 33.7 percent for P. ostreatus and 32.8 percent for F. velutipes, respectively, compared to conventional spawn making system. The system of liquid spawn inoculation was successfully operated without malfunction in opening or closing the lid, and it took 26 min to inoculate 1200 bottles.