Chemical composition, antioxidant and antimicrobial activity of guavirova (Campomanesia xanthocarpa Berg) seed extracts obtained by supercritical CO2 and compressed n-butane

摘要

This work reports the chemical characterization, antioxidant and antimicrobial activity of Campomanesia xanthocarpa seed extracts obtained from compressed n-butane and supercritical CO2 extraction. For supercritical CO2, the extraction condition was 40 degrees C and 250 bar, while for n-butane 35 degrees C and 10 bar. The total phenolic compound content was 68.58 +/- 1.15 mg g(-1) for n-butane extract and 17.18 +/- 2.44 mg g(-1) for supercritical CO2. Both extracts were rich in terpenoids, especially beta-caryophyllene, while n-butane extract had a larger content of flavonoids than supercritical CO2. The antioxidant activity was tested with FRAP, DPPH and deoxyribose assay, with n-butane extracts exhibiting better results. Antimicrobial activity was verified for both extracts against Pseudomonas aeruginosa, however, supercritical CO2 extract afforded a better performance, reaching 5.0 mg mL(-1) for MIC and 10.0 mg mL(-1) for MBC, while n-butane extract showed 40.0 mg mL(-1) in both antimicrobial tests. Higher extraction yields were obtained using n-butane, which also presented higher antioxidant activity, while supercritical CO2 extracts afforded the highest antimicrobial activity. (C) 2015 Elsevier B.V. All rights reserved.