Evaluation of the durability and antiadhesive action of 2-methacryloyloxyethyl phosphorylcholine grafting on an acrylic resin denture base material

摘要

Statement of problem The polymer 2-methacryloyloxyethyl phosphorylcholine is currently used on medical devices to prevent infection. Denture plaque-associated infection is regarded as a source of serious dental and medical complications in the elderly population, and denture hygiene, therefore, is an issue of considerable importance for denture wearers. Furthermore, because denture bases are exposed to mechanical stresses, for example, denture brushing, the durability of the coating is important for retaining the antiadhesive function of 2-methacryloyloxyethyl phosphorylcholine. Purpose The purpose of this study is to investigate the durability and antiadhesive activity of two 2-methacryloyloxyethyl phosphorylcholine polymer coating techniques: poly-2-methacryloyloxyethyl phosphorylcholine grafting and poly-2- methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate coating. It was revealed that 2-methacryloyloxyethyl phosphorylcholine polymer coating of the denture base resin polymethyl methacrylate decreases bacterial biofilm formation. Material and methods Durability was examined by rhodamine staining and elemental surface analysis and by determining the wetting properties of the 2-methacryloyloxyethyl phosphorylcholine polymer-modified polymethyl methacrylate after a friction test that comprised 500 brushing cycles. Antiadhesive activity was examined by using a Streptococcus mutans biofilm formation assay. Results Poly-2-methacryloyloxyethyl phosphorylcholine-grafted polymethyl methacrylate retained 2-methacryloyloxyethyl phosphorylcholine units and antiadhesive activity even after repetitive mechanical stress, whereas co-n-butyl methacrylate-coated polymethyl methacrylate did not. Conclusion These results demonstrated that graft polymerization of 2-methacryloyloxyethyl phosphorylcholine on denture surfaces may contribute to the durability of the coating and prevent microbial retention.