PREPARATION OF BETA-CYCLODEXTRIN SULFATE-IMMOBILIZED HYDROPHILIC VINYL-POLYMER GEL AS A SELECTIVE, HIGH RECOVERY AND STABLE ADSORBENT FOR HIGH-PERFORMANCE AFFINITY CHROMATOGRAPHY OF HEPARIN-BINDING SUBSTANCES

摘要

beta-Cyclodextrin sulfate having heparin-mimicking activity was immobilized on a hydrophilic vinyl-polymer gel, TSKgel AF-Epoxy, TOYOPEARL 650M. A column packed with this material could be used for high-performance affinity chromatographic separation of heparin-binding substances such as growth factors, enzymes, coagulation factors and lipoproteins. The substances retained on the column were recovered more easily than those on a heparin-immobilized column. Furthermore, the beta-Cyclodextin sulfate-immobilized gel was superior in stability to the heparin-immobilized gel when exposed to acidic and basic solutions. [References: 16]