Multi-Residue Method for the Screening of Benzimidazole and Metabolite Residues in the Muscle and Liver of Sheep and Cattle Using HPLC/PDAD with DVB-NVP-SO3Na for Sample Treatment

摘要

A high-performance liquid chromatography (HPLC) screening method with a photodiode array detector (PDAD) was established for the simultaneous determination of residues of 13 benzimidazoles (BZDs) and metabolites in sheep and cattle muscle and liver. Samples were extracted by ultrasonication in ethyl acetate and purified over DVB-NVP-SO3Na sorbent. Under the optimized conditions, good linearities were obtained for BZDs and metabolites with correlation coefficients (R-2) greater than 0.9911. The recoveries of the 13 BZDs and metabolites from spiked samples were 72.0-119.3 %, with intraday and interday relative standard deviations (RSDs) below 22.8 %. The limits of detection (LODs) and quantitation (LOQs) were 0.8-4.9 and 2.6-18.2 mu g kg(-1), respectively. The results clearly demonstrated that the developed approach enables reliable screening of 12 BZDs and metabolites except flubendazole (FLU) and could be used as a regulatory tool for the screening of BZD and metabolite residues in the muscle and liver of sheep and cattle.