Effect of insulin-dependent cytoplasmic regulator on murine melanoma kml cell proliferation

摘要

Insulin-dependent cytoplasmic regulator (IDR), the activity of which correlates with the insulin level in blood, was isolated from rat liver and fractionated on a Sephadex G-25 column. Radioautography established that IDR stimulates by 30% proliferation of murine melanoma KML cells in culture. This is indicated by ~3H-thymidine incorporation into the cell nucleus. It is proposed that insulin stimulates proliferation of the melanoma KML cell cull cultures through an intracellular intermediate, IDR.