Metabolites from the Marine Isolate of the Fungus Aspergillus versicolor KMM 4644

摘要

In continuation of the search for biologically active compounds in extracts of marine isolates of mycelial fungi, we isolated the strain Aspergillus versicolor KMM 4644 from sediment of Sakhalin Bay, Sea of Okhotsk. The fungus was cultivated on solid medium for 21 d at room temperature in 20 Ehrlenmeyer flasks (500 cm~3), each of which contained medium of the following composition: rice, 15.0 g; yeast extract, 0.02 g; KH_2PO_4, 0.01 g; sodium tartrate, 0.01 g; seawater, 20 mL. Mycelium with medium was extracted with EtOAc. The extract was evaporated in vacuo. The dry residue (3.15 g) was chromatographed over a column of silica gel using hexane:EtOAc mixtures of increasing polarity. A fraction (hexane:EtOAc, 70:30) was separated by HPLC over a Diaspher-110-C_(18) column using H_2O:MeOH (50:50) to isolate 1 (12 mg), 2 (2.5 mg), and 3 (7 mg). Separation of another fraction (hexane:EtOAc, 95:5) using the same column and H_2O:MeOH (85:15) afforded 4 (2 mg) and 5 (6.5 mg).