Validated TLC-Image Analysis Method for Simultaneous Quantification of Curcuminoids in Curcuma longa

摘要

A simple and rapid thin layer chromatographic (TLC)-image analysis method was developed for simultaneous quantification of three curcuminoids; curcumin (CUR), desmethoxycurcumin (DES) and bisdesmethoxycurcumin (BIS), in Curcuma longa (turmeric). Chromatographic separation of the curcuminoids was achieved on silica gel 60 F254 TLC plates, using chlo-roform-hexane-methanol (1:1:0.1, v/v/v) as the mobile phase. Image analysis of the scanned TLC plate was performed by Photoshop 7.0 to quantify the amount of each curcuminoid. The method was validated and found to be accurate, reliable and convenient for the analysis of CUR, DES and BIS in turmeric.