A Comparison of Three Methods of Extraction for the Determination of Polyphenols and Organic Acids in Tobacco by UPLC-MS-MS

摘要

Matrix solid phase dispersion (MSPD), ultrasonic extraction followed by a solid phase extraction (USE-SPE) and reflux extraction (REFLUX) were studied for the analysis of polyphenols and organic acids in tobacco. The analysis was by ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS-MS). The multi-mode support sorbent Zirconia/AA12S50 in MSPD is more suitable for the extraction of tobacco polyphenols than conventional silica or C18 silica. Although the matrix effect of USE-SPE is slightly stronger than MSPD and REFLUX for most target compounds, it gave higher extraction capacity, recoveries and sensitivity.