Catalytic and DNA-hydrolyzing activities of purified immunoglobulins IgG from patients sera with autoimmune disease by pseuclobiospecific chromatography using histicly-aminohexyl-sepharose

摘要

Catalytic autoimmune antibodies from patients with antiphospholipid (aPL) antibodies were purified using histidyl-aminohexyl-sepharose gel. The sera were loaded on the columns equilibrated with 25 mM MOPS buffer pH 7.4 and the absorbed proteins were eluted by adding 0.2 M NaCl in the equilibrating buffer. Antibodies purity was evaluated by SDS-PAGE. The purified immunoglobulins G from patients with (aPL) sera by histidyl-aminohexyl-sepharose ;show DNA-degrading activity of the plasmid pUC19 DNA and catalytic activity in hydrolyzing the pepticle substrate Pro-Phe-Arg-7-amido-4-methylcoumarin.