Simultaneous analysis by high performance liquid chromatography of hydrophilic compounds and lipophilic compounds (alkamides) from pharmaceutical preparations of Echinacea purpurea root

摘要

The two major classes of compound (hydrophilic and lipophilic) from Echinocea purpureo L. Moench, responsible for the immunostimulating effect of the plant, have usually been determined by two different methods. This paper describes simultaneous analysis by high-performance liquid chromatography (HPLC) of hydrophilic compounds and lipophilic compounds (alkamides) from pharmaceutical preparations of Echinacea purpureo root. A linear binary gradient, at a flow of 1 mL min(-1), starting with 1:9 acetonitrile-water (both containing 0.1% orthophosphoric acid) and ending with 8:2 acetonitrile-water (both containing 0.1% orthophosphoric acid) after 50 min, was optimized for separation of the compounds. Hydrophilic compounds were separated in the first 25 min and the lipophilic compounds between 25 and 50 min. The compounds were identified on the basis of correlation of retention times and spectral data. The external standard method was used for quantitative analysis. [References: 5]