Procedure for the Determination of Biogenic Amines by Derivatization with Dabsyl Chloride and Reversed Phase Liquid Chromatography

摘要

Existing procedrues for the determination of biogenic amines by drivatization with dabsyl chloride and separation by RPLC differ very little from those employed in omino acid analysis. Since some difficulties attributable to the higher hydrophobicity of dabsyl-amides were detected on using these procedures, important modifications are proposed, including extriaction of the reaction mixture with an orgaic solvent, drying of the exttract and redissolving the residue in acetone before injecting. Dabsylation and chromatographic conditions were also studied. Athough recovery from spiked meat extracts is very good, significant matrix effects are detected when extraction is carried out from spiked whole meat samples. These adverse effects cn be overcome by means of standard addition.dverse effects can be