Chiral ligand-exchange chromatography on an RP HPLC column coated with a new chiral selector derived from L-spinacine

摘要

A commercial reversed-phase (RP) C,8 HPLC column has been dynamically coated with the chiral selector N-T-n-decyl-L-spinacine and then loaded with copper(II) ions. Several racemic mixtures of underivatized amino acids and oligopeptides were resolved on the column by chiral ligand-exchange chromatography The most important experimental conditions affecting column efficiency, retention, and selectivity (temperature and mobile phase flow rate and composition) were extensively investigated. [References: 27]