An original way to use a beta-cyclodextrin-bonded silica stationary phase in electrochromatography. Application to the achiral separation of nucleobases and nucleosides

摘要

Although beta-cyclodextrin-bonded silica stationary phases are usually dedicated to the separation of enantiomers, they can also be used for achiral purposes, taking advantage of the complex interactions between the solutes and the cyclodextrins. The work described in this paper was intended to show how such stationary phases can be used in short-end injection capillary electrochromatography (CEC) for rapid (< 6 min) resolution of a mixture of purines and pyrimidines. Several experimental conditions (mobile-phase composition, voltage and temperature) were investigated to determine the optimum operating conditions for the method, which can also be applied to the analysis of nucleosides. Quantitative analysis was also performed; theophylline was used as internal standard to assess the linearity and the repeatability of the method. Limits of detection ranged from 4 to 13 μM and repeatability was good (RSD never exceeded 2.6%). [References: 35]