HPLC determination of berberine in plasma of patients with ischemic heart failure

摘要

Berberine absorption by patients with ischemic heart failure (IHF) after oral administration and the relationship between clinical effect and plasma berberine concentration are studied. Plasma samples were pretreated by chloroform extraction. Berberine was determined on a mu Bondapak column with acetonitrile-phosphoric acid mobile phase and UV detection. The limit for berberine in plasma was 8 ng mL(-1) for an injection volume of 50 mu L. Average berberine recovery was 96.5 %. Results showed that improvements in symptoms were more significant for patients with plasma berberine concentration > 0.1 mg L-1 than for those with < 0.1 mg L-1. Plasma berberine monitoring may be helpful in the treatment of patients with IHF. [References: 3]