Henriciella marina gen. nov., sp. nov., a Novel Member of the Family Hyphomonadaceae Isolated from the East Sea

摘要

A bacterial strain, designated Iso4~T, was isolated from the East Sea of Korea and was subjected to a poly-phasic taxonomy study including phenotypic and chemotaxonomic characteristics as well as 16S rRNAgene sequence analysis. Cells of the strain were Gram-negative, motile, non-budding, non-stalked, andstrictly aerobic. Strain Iso4T grew optimally at 20C in the presence of 1-2% (w/v) NaCI and at pH 6.9-7.6. The major respiratory quinone was Q-10 and the major cellular fatty acids were C1e:1 ω7c (53.5%),C17:1 ω5c (11.7%), C17:1ω6c(8.1%),C16:0(7.8%),C17:0(4.8%),Cis:0(2.9%),andC16:1ω5c (2.2%). The DNA G+C content of strain Iso4T8.1 was 56.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequencesshowed that strain Iso4T formed a monophyletic clade in the family Hyphomonadaceae, supported by highbootstrap value and was most closely related to the genus Hyphomonas (9294%), a member of marinebacteria in the family. The phenotypic, genotypic, and chemotaxonomic evidences also suggest strain Iso4Trepresents a novel genus and species in the family Hyphomonadaceae for which the name Henriciella gen.nov., sp. nov. is proposed. The type strain is Iso4T (=KCTC 12513T=DSM 19595T=JCM 15116T).