首页> 外文期刊>The journal of microbiology >The Influence of NaCl and Carbonylcyanide-m-Chlorophenylhydrazone on the Production of Extracellular Proteases in a Marine Vibrio Strain
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The Influence of NaCl and Carbonylcyanide-m-Chlorophenylhydrazone on the Production of Extracellular Proteases in a Marine Vibrio Strain

机译:NaCl和羰基氰化物-间氯苯hydr对海洋弧菌菌株细胞外蛋白酶产生的影响

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In general, the salinity of the ocean is close to 3.5% and marine vibrios possess the respiratory chainlinked Na+ pump. The influence of sodium chloride and the proton conductor carbonylcyanide m-chlorophenylhydrazone (CCCP) on the production of extracellular proteases in a marine Vibrio strain was examined. At the concentration of 0.5 M, sodium chloride minimally inhibited the activity of extracellular proteases by approximately 16%, whereas at the same concentration, the producton of extracellular proteases was severely inhibited. On the other hand, the production of extracellular proteases was completely inhibited by the addition of 2 μM CCCP at pH 8.5, where the respiratory chain-linked Na~+ pump functions.
机译:通常,海洋的盐度接近3.5%,海洋弧菌拥有呼吸链式Na +泵。检验了氯化钠和质子导体羰基氰化物间氯苯基((CCCP)对海洋弧菌菌株中细胞外蛋白酶产生的影响。在0.5 M的浓度下,氯化钠对细胞外蛋白酶的抑制作用最小,约为16%,而在相同的浓度下,细胞外蛋白酶的产生受到严重抑制。另一方面,在pH 8.5处添加2μMCCCP可以完全抑制细胞外蛋白酶的产生,在该处呼吸链连接的Na〜+泵起作用。

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