Isolation and Characterization of α-Glucosidase Inhibitor from the Fungus Ganoderma lucidum

摘要

An α-glucosidase inhibitor, SKG-3, was isolated from the fruiting bodies of Ganoderma lucidum and its physico-chemical properties were characterized. It was a highly specific and effective reversible inhibitor of α-glucosidase. It showed very potent inhibitory activity against α-glucosidase with an IC_(50) value of 4.6 μg/ml, but no activity for any other glycosidases tested. Enzyme activity could be recovered upon dialysis, thus providing evidence for the reversibility of the inhibition. A Linevveaver-Burk plot indicated that the SKG-3 inhibition of α-glucosidase was competitive.