Enhanced Secretion of Cell Wall Bound Enolase into Culture Medium by the sool-1 Mutation of Saccharomyces cerevisiae

摘要

In order to identify the protein(s) secreted into culture medium by the sool-1/retl-1 mutation of Saccharomyces cerevisiae, proteins from the culture medium of cells grown at permissive (28℃) and non-permissive temperatures (37℃), were analyzed. Comparison of protein bands separated by SDS-PAGE identified a prominent band of 47-kDa band from a mutant grown at 37℃. N-terminal amino acid sequencing of this 47-kDa protein showed high identity with enolases 1 and 2. Western blot analysis revealed that most of the cell wall-bound enolase was released into the culture medium of the mutant grown at 37℃, some of which were separated as those with lower molecular weights. Our results, presented here, indicate the impairment of cell wall enolase biogenesis and assembly by the sool-1/retl-1 mutation of S. cerevisiae.