A bicyclo[3.2.1]octanoid neolignan and toxicity of the ethanol extract from the fruit of Ocotea heterochroma

摘要

A new bicyclo[3.2.1]octanoid neolignan rel-(7S,8R,1'S,2'R,3'S)-Delta 8'-2'-hydroxy-5,1',3'-trimethoxy-3,4methylenedioxy-7,3',8,1'-neolignan (1) was isolated from ethanol extract from the fruit of Ocotea heterochroma Mez & Sodiro ex Mez as well as the known compounds beta-friedelanol (2), meso-dehydroguaiaretic acid (3), and yangambin (4), whose structures were elucidated on the basis of their comprehensive spectroscopic analysis including 2D NMR data. Lethality bioassay using brine shrimp (Artemia salina Leach) was evaluated with the ethanol extract from the Ocotea heterochroma's fruit. The toxicity of this extract was greater than the toxicity of those fractions obtained in a first solvent partition (benzene, ethyl acetate, and butanol subfractions) and that of a mixture of acetylated 2'-epimers from the new neolignan 1.