Klebsiella alba sp. nov., a novel pesticide-tolerant bacterium fror a heavily polluted environment

摘要

A novel Gram-negative, aerobic, rod-shaped and pesticide (chlorpyrifos and atrazine) tolerant strain, designated CW-D 3~T, was isolated from a heavily polluted soil sample in Jiangsu Province, China. The strain could tolerate about 800 μg·ml~(-1) atrazine or chlorpyrifos when added in TYB medium. A polyphasic taxonomy including phylogenetic and phenotypic analysis was performed on the new isolate. Phylogenetic analysis and chemotaxonomic characteristics revealed that strain CW-D 3~T belongs to the genus Klebsiella. Its closest phylogenetic neighbors were K. singaporensis (strain LX3~T), three subspecies of K. pneumoniae (strains ATCC 13883~T, ATCC 13884~T and ATCC 11296~T) and K. granulomatis, according to 16S rRNA and rpoB gene analysis. The 16S rRNA gene sequence similarity was about 94.8-97.6% and the rpoB gene sequence similarity was about 96.8-98.6% to its phylogenetic neighbors. Strain CW-D 3~T also showed low DNA-DNA reassociation values (less than 21%) with respect to the three subspecies of K. pneumoniae and Klebsiella singaporensis. Its major fatty acids were C_(14:0), C_(16:0), c-C_(17:0) and c-C_(19:0) ω8c. The G+C content of the DNA was 58.3 mol%. Phylogenetic and phenotypic results supported the assignment of CW-D 3~T as a novel species of the genus Klebsiella, for which the name Klebsiella alba sp. nov. is proposed. The type strain is CW-D 3~T (=LMG 24441 ~T=KCTC 12878~T =CCTCCAB 206144~T).