A novel ribonuclease from the veiled lady mushroom Dictyophora indusiata.

摘要

A ribonuclease (RNase), exhibiting a molecular mass of 28 kDa and specificity toward polyU and polyA and possessing an N-terminal sequence dissimilar to previously reported mushroom RNases, was isolated from dried fruiting bodies of veiled lady mushroom (Dictyophora indusiata). It demonstrated an RNase activity of 564 U/mg toward yeast transfer RNA. The RNase was adsorbed on DEAE-cellulose, CM-Sepharose, and Q-Sepharose. It demonstrated a pH optimum of 4–4.5 and a temperature optimum of 60 degrees C. There was a loss of RNase activity at temperatures above 60 degrees C.