Lentiviral transduction of human hematopoietic cells by HIV-1-and SIV-based vectors containing a bicistronic cassette driven by various internal promoters

摘要

Background Lentiviral gene transfer into hematopoietic cells has been mostly optimized with vectors carrying a single reporter gene. For many clinical applications, lentiviral vectors should contain more than one gene because transduced cells should be enriched by a selectable marker or killed for safety reasons after use. Thus, we compared various vectors containing a bicistronic cassette driven by different ubiquitous promoters for their ability to transduce human T-lymphocytes, CD34(+)-cells, and dendritic cells (DCs) derived from CD34(+)-cells or monocytes.