Surface-Enhanced Raman Scattering of Single Adenine Molecules on Silver Colloidal Particles

摘要

To find feasibility of identifying nucleotides at the single-molecule level, we implemented Raman imaging and spectroscopy of adenine molecules adsorbed on Ag colloidal nanoparticles. Surface enhanced Raman scattering (SERS) of adenine molecules showed an intermittent on-and-off behavior called "blinking". The observation of blinking provides substantial evidence for detecting single adenine molecules.