The lipophilic yeast, genus Malassezia is a part of the normal cutaneous microflora of human and warm-blooded vertebrates. Species of the genera were re-classified to seven species; M. pachydermatis, M. globosa, M. furfur, M. obtusa, M. restricta, M. slooffiae and M. sympodialis. However, the means of species identification in conventional clinical laboratories have not been reported and the clinical significance of each species is not clearly understood. Species identifications of genus Malassezia which depend on the morphological, physiological characters are difficult and time-consuming. Recently, many molecular techniques have been developed for identification or typing of Malassezia. PCR-mediated methods, PCR-direct sequencing and nested-PCR using specific primers, are useful to identify the spices. The basic information obtained from these approaches have been contributing to the understanding of these pathogenic yeasts and related diseases.
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机译:亲脂酵母,马拉色霉菌属是人类和温血脊椎动物正常皮肤微生物区系的一部分。属的种类被重新分类为七种; M. pachydermatis,M。globosa,M。furfur,M。obtusa,M.stricta,M。slooffiae和S. sympodialis。但是,尚未报道常规临床实验室中物种鉴定的方法,并且每种物种的临床意义还不清楚。依赖于形态,生理特征的马拉色菌属的种类鉴定是困难且耗时的。近来,已开发出许多用于鉴定或鉴定马拉色菌的分子技术。 PCR介导的方法,PCR直接测序和使用特定引物的巢式PCR可用于鉴定香料。从这些方法获得的基本信息有助于理解这些致病性酵母菌和相关疾病。
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