Affnity CapillaryElectrophoresis in a Poly(dimethylsiloxane)-glass Hybrid Microchip

摘要

Affinity capillary electrophoresis of DNA was conducted in a poly(dimethylsiloxane)-glass hybrid microchip with a 100mum (width)X130 mum(height)microchannel containing poly(N,N-dimethylacrylamide)-DNa conjugate.By this method,sample 12-mer oligonucleotide composed of c-K-ras codon 10-13 was separated form its single-base substituted mutant within 2 min.