Giemsa and Centromere Banding Patterns in the Chromosomes of Indian Donkey (Equus asinus)

摘要

The individual chromosome morphology of Indian domestic donkeys was studied by karyotyping and banding techniques. Blood samples were collected from 20 domestic donkeys (5 males and 15 females) inhabited in four districts of Tamil Nadu, India. Chromosome preparations were made from short-term lymphocyte culture technique. A total of 342 G-bands were recorded in a haploid set of chromosomes. The C-banding profile revealed presence of darkly stained centromeric C-bands in chromosomes 1, 2 and 6 and inall acrocentric chromosomes. Chromosomes lp, 3p, 6q, 8p, l0p, lip, 12p, 13q, 16p, 17p, 18p, 20p, 22p, and 23p exhibited telomeric heterochromatin. The Y chromosome consisted entirely of constitutive heterochromatin and the X chromosome lacked centromeric heterochromatin but had an interstitial C-band.