Evaluation of field diagnostic tests for the detection of Newcastle disease virus.

摘要

258 tissue samples collected from poultry during field outbreaks of Newcastle disease (ND) in India were examined to compare the efficacy of 5 diagnostic tests. 180 samples were positive by haemagglutination and haemagglutination inhibition test (standard HA and HI tests, 69.76%), 165 samples by latex agglutination test (LAT, 63.95%) 175 samples by avidin biotin-dot ELISA (AB-Dot-ELISA, 67.8%) and 157 samples by immunoperoxidase test (IPT, 60.85%). The sensitivity and specificity were 98.28% and 92.86% for Dot-ELISA, 97.63% and 87.64% for LAT, 99.4% and 95.12% for AB-Dot-ELISA and 95.12% and 84.78% for IPT. The only significant difference in results was between those of IPT and HA and HI tests. Dot-ELISA and LAT are recommended for diagnosing ND in field conditions because they are less costly than AB-Dot-ELISA.