Molecular Studies on Theileriosis and Identification of Theileria OrientalisIndia Using PCR

摘要

The study comprised of 12 clinical cases of Theileriosis in cattle and 30 apparently healthy cross bred cattle which were subjected to PCR screening for Theileria annulata and Theileria orientalis. The amplicons of Theileria annulata and Theileria orientalis were subjected to automated sequencing. The twelve clinical cases of Theileriosis which were initially confirmed by blood smear examination and later subjected to PCR revealed T. orientalis in 8 cases, T. annulata in 3 cases and combined infection in 1 case. All the blood smear samples of apparently healthy cattle were found negative for Theileriosis. However, PCR screening of the blood samples of these cattle revealed 11 positive for Theileria sp. infection comprising 5 T. orientalis 3 T. annulata and 3 combined T.orientalis and T. annulata. Theileriosis in India is mainly thought to be caused by T. annulata. However, in this study, T.orientalis was identified as the predominant causative agent of Theileriosis.