Effect of Age and Scrotal Circumference on Sperm Motion Characteristics of Frozen-Thawed Semen in Breeding Buffalo Bulls Measured by the Computer-Assisted Semen Analyzer

摘要

Thirty Murrah buffalo bulls were randomly selected from two government bull farms. On the basis of age, these bulls were divided into mature (48 - 83 months; n = 17) and old (84 -107 months; n = 13). Based on the measurement of scrotal circumference,they were grouped into two viz. group I (36.0 - 39.0 cm, n = 15) and group II (> 39.0 cm, n = 15). Thirteen different sperm motion characters were measured by the computer-assisted semen analyzer (CASA) in the frozen-thawed of each bull. It was observedthat mature bulls compared to old bulls had significantly (P < 0.05) high VSL (89.0 ± 2.4 vs 80.9 ± 4.0 um/s), VCL (175.5 ± 4.9 vs 158.6 ± 5.8 fim/s) and RM (36.1 ± 2.5 vs 28.8 ± 3.3 %). Similarly, bulls in group I had significantly (P < 0.05) highVAP (108.9 ± 3.6 vs 98.5 ± 4.6 um/s), VSL (90.2 ± 2.6 vs 80.8 ± 3.4 um/s) and VCL (177.9 ± 5.2 vs 158.4 ± 5.0 um/s) than in group II. Briefly, the mature bulls and group I bulls exhibited better CASA-determined post-thaw spermiogram as compared toold bulls and group II bulls.